Part:BBa_K2578511:Design

Protein coding sequence for Saccharomyces cerevisiae metallothionein 1 (CUP1)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The DNA sequence was modified to suit the synthesis by IDT while retaining the original amino acid sequence.

Source

NCBI

References

[1] NCBI gene ID 856450

[2] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327

[3] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.

[4] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[5] Brenes-Pomales, A., Lindegren, G., & Lindegren, C. C. (1955). Gene Control of Copper-Sensitivity in Saccharomyces. Nature, 176(4487), 841-842. doi:10.1038/176841a0

[6] Adamo, G., Brocca, S., Passolunghi, S., Salvato, B., & Lotti, M. (2012). Laboratory evolution of copper tolerant yeast strains. Microbial Cell Factories, 11(1), 1. doi:10.1186/1475-2859-11-1

[7] Berka, T., Shatzman, A., Zimmerman, J., Strickler, J., & Rosenberg, M. (1988). Efficient expression of the yeast metallothionein gene in Escherichia coli. Journal of Bacteriology, 170(1), 21-26. doi:10.1128/jb.170.1.21-26.1988